BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3428

Peptides:

Sequences	Affinity Values
GQSEHHMRVASF(60/67) GLHTSATNLYLH(3/67) STPIFAEATARS(2/67) SGVYKVAYDWQH(1/67) ALKTHSVSPAPR(1/72)	[0.42] [0.76] [1.20] [0.33] [1.11]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 30496775

Target Name:

Receptor tyrosine-protein kinase erbB-2

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

The absorbance at 450 nm was measured after the reaction with the HRP-anti-M13 antibody conjugate (diluted 1:2000 in the blocking buffer). A450 was reproduced from Fig. 2 and shown.

Brief Description:

Biopanning was performed as follows: 1. Immobilization of HER2 to Ni-NTA magnetic particles (MPs); 2. Phage binding; 3. Washing and elution of bound phage; 4. Amplification; 5.Two negative selection steps (one against BSA-blocked MPs and another against Ni-NTA MPs).

BiopanningDataSet Comments:

Curator:

Bifang He

Curation Date:

2019-05-28

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