BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3422

Peptides:

Sequences
CIWVDFRWKC(4) CVWVDFHWVC(2) CVWVSFHWVC(2) CFWWKYVYRC(1) CVYHGFRWRC(1) CRYIDFRWSC(1) CFWYGMRWWC(1) CFWYSYRWIC(1) CRYSAWKWWC(1) CWRALNYSPC(1) CGSDGEVGRC(1)

Sequences Features:

Motif:

FRW

Unique Sequence Number:

Experimental Method:

Phage display (in vivo)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 30670660

Target Name:

Cerebellum

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

fUSE55-based CX8C phage display library (CX8C)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

To isolate peptides targeting the brain, animals received the CX8C phage library by i.v. injection. After 30 minutes in circulation, mice were perfused with 20 mL of Dulbecco’s modified Eagle’s medium (DMEM) and different regions of the brain (cerebellum, olfactory bulb and hemispheres) were collected. Phage bound to tissue were recovered by tissue homogenization followed by bacterial infection, transferred to LB media supplemented with kanamycin (100 μg/ml) and tetracylcin (20 μg/ml) amplified by overnight culture and purified from culture supernatants by the PEG/NaCl method. Two more successive rounds of selection were then performed by reinjecting i.v. the recovered pools of phage into different mice (one for each brain area).

BiopanningDataSet Comments:

Peptides containing a conserved motif in which amino acids Phenylalanine−Arginine−Tryptophan (FRW) predominate could be visualized by transmission electron microscopy bound to the junctions between endothelial cells in all areas of the brain, including the optic nerve, but not in other barrier-containing tissues, such as intestines and testis. Remarkably, peptides containing the motif do not bind to vessels in the retina, implying an important molecular difference between these two vascular barriers. Furthermore, the peptide allows for in vivo imaging, demonstrating that new tools for studying and imaging the brain are likely to emerge from this motif.

Curator:

Bifang He

Curation Date:

2019-05-23

First |

Prev |

3422

| Next

| Last