BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3418

Peptides:

Sequences
LPPWKLK(11/27) WSLSELH(4/27) LHRHANL(2/27) IGASVHR(1/27) AHHLKVS(1/27) NHPLYNR(1/27) ALEHTSR(1/27) HPAIRPP(1/27) SSNQFHQ(1/27) KVPGHQQ(1/27) TLAPRTA(1/27)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 30889924

Target Name:

Polycrystalline SnO powder

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

For each biopanning procedure, 20 mg of the SnO powder were equilibrated for 10 min in 1 mL Tris-buffered saline + 0.1 % Tween-20 (TBST0.1) in an ultrasonic bath. After centrifugation at 10621 g for 2 min followed by removal of the supernatant, the SnO powder was resuspended in 200 μL TBST0.1. 10 μL of the phage peptide library (~1.2e11 phages) were added for 1 h under light agitation at 20 °C. To remove unbound and weakly bound phages, the SnO powder was washed 10 times with 1 mL TBST0.5. For this, first the powder was sedimented by centrifugation at 10621 g for 2 min. Subsequently, the supernatant was removed and the powder resuspended in 1 mL TBST0.5 by gentle vortexing, followed by another centrifugation step. Bound phages were eluted by 1 mL of 0.2 M Glycine-HCl (pH 2.2) supplemented with 1 mg/mL BSA. After 10 min of gentle mixing, the powder is sedimented by centrifugation, and the supernatant is transferred to a new 1.5 mL tube and neutralized by adding 150 μL of 1 M Tris–HCl (pH 9.1). The obtained M13 phages were amplified in E. coli ER2738, according to manufacturer’s recommendations.

BiopanningDataSet Comments:

Curator:

Bifang He

Curation Date:

2019-05-23

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