BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3415

Peptides:

Sequences

RVRSWRVRR(2)
LVRPRAGRS(1)
PRALRSVVR(1)
PRAMRGGRR(1)
PRGMRVVQR(1)
TPRSARFGD(1)
TLMVPRTGS(1)
PRSWWKAAW(1)
LVYMPRAFL(1)
RSARFKSIW(1)
RAAKSGLHF(1)
RAFRWGRAP(1)
RSRSSRLKV(1)
RSSRVTSRM(1)
SLRIRSSRS(1)
LRLKLDIGR(1)
RRQCRALRY(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

ABI PRISMH 3700 DNA Analyzer

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 22384068

Target Name:

Prothrombin

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

X9 T7 phage display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

In brief, phages are anchored to nickel nitrilotriacetic acid (Ni-NTA) beads via the His6-tags before the first protease treatment. The protease is added and allowed to react over night, releasing phages displaying cleavage-susceptible 9-mers from the beads. Samples are centrifuged and cleaved phages are collected in the supernatant. These phages are amplified in the E. coli strain BLT5615 and enter the next selection round (biopanning). After five biopannings with 1 U of thrombin, enriched cleavage-susceptible phages are sequenced.

BiopanningDataSet Comments:

Curator:

Bifang He

Curation Date:

2019-05-23

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