BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3401

Peptides:

Sequences
YCHPQFCG(4) HCHPQFCS(3) LCHPQFCG(2) DCHPQFCS(2) NCHPQFCP(1) DCHPQFCR(1) DCHPQFCV(1)

Sequences Features:

Motif:

HPQ

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 8508957

Target Name:

Streptavidin

Template Name:

Biotin

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

TN2 phage display library (XCX4CX)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Library fractionation involved a 2-h incubation of phage with target, ten rapid washes with PBS containing 0.1% Tween-20 (to remove unbound phage), and stepwise washes with citrate buffers of pH 7, 6, and 5. Bound phage were removed from target-coated wells by the addition of pH 2 citrate-buffered elution solution for 10 min followed by immediate neutralization with Tris pH 8 buffer to a final concentration of 250 mM Tris. A portion of the phage eluted at pH 2 was used to grow a fresh stock of display phage which was then further fractionated. This sequence of events constitutes a single round of enrichment and amplification.

BiopanningDataSet Comments:

Curator:

Bifang He

Curation Date:

2019-05-17

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