BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3395

Peptides:

Sequences

Affinity Values

KRLTPLSPDHYD(14/30)
QLKLHNKGLISS(1/30)
GWSSREASQEGN(1/30)
NGVFAMLFPMQS(1/30)
YGILARDNDSLG(1/30)
VAYEGSYFAVYQ(1/30)
SIDSGYRPGVRY(1/30)
ADHWWSYAYQKP(1/30)
VVVSSLSAPYKD(1/30)
VPAPPISSASD(1/30)
ITTPDWELMRGM(1/30)
GIVEPSDRSTRG(1/30)
AFDSPDNPVASN(1/30)
EYHDYESANAAD(1/30)

[4.8 ± 1.0]
[3.2 ± 1.5]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 30344835

Target Name:

Ovarian cancer tissue slice

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Flow Cytometry

Affinity Measurement Description:

Peptides were synthesized (>98% purity) and conjugated with FAM (excitation wavelength = 490 nm, emission wavelength = 525 nm) at the 5'- and carboxyl termini (Protech Co., Ltd., Taiwan). These fluorescently-labeled peptides were then used for fluorescence microscopy analysis and flow cytometric assays (BD Accuri™ C6, Becton, Dickinson and Company, USA). The resulting fluorescence intensity of the affinity reagents was further used to calculate the equilibrium dissociation constant (Kd, nM) with Prism software (GraphPad Software, USA).

Brief Description:

Selection was performed against ovarian cancer tissue slides by the integrated microfluidic system capable of automating SELEX and phage display technology. Briefly, an M13 phage library was incubated with normal tissues (“negative selection” step), and then the unbound M13 phages were incubated with cancer tissues (“positive selection” step). There were three rounds of both positive and negative panning involved for the entire screening process.

BiopanningDataSet Comments:

Curator:

Yuwei Zhou

Curation Date:

2019-01-29

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