BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3378

Peptides:

Sequences

GWSLFLEVL(3)
GMATFLELH(3)
AVLLFAGIL(3)
WEVMFLDFH(2)
MSLFWSDWH(2)
WSVLFNWGH(2)
TPGGYILML(2)
GWGVFLDVG(1)
GGVLFLDYF(1)
GQALFLDIF(1)
LIMLFLEEE(1)
GIVGFLEVL(1)
GWEFFLDAE(1)
GVVSFLEMC(1)
GWVSFLEPR(1)
RATSFLEWH(1)
VVMSFLDFS(1)
GPVLWLEVM(1)
AWVPWLDLV(1)
GWVSYLEGG(1)
GWMLYLEWR(1)
LFVNFGEHH(1)
WYVYFGELD(1)
GATTFTDMV(1)
GLETFIEVV(1)
TPGGFVELY(1)
WMTSFSDVM(1)
WVVSFSETP(1)
GIVVWSDVL(1)
SVELWSEVL(1)
AWVPWSEVH(1)
VERFWSVEH(1)
PVLLFMDFP(1)
GGVLFMDEI(1)
GWSAFMEFT(1)
DTRFFQEWL(1)
GFLSFWDLA(1)
TWVSFFDLH(1)
GVISFFEFV(1)
GWVTWWDIG(1)
EWMTYWEEG(1)
EVIKWWDFH(1)
GVVQYWGGD(1)
WGVFYWEHH(1)
GYALWWAIE(1)
GWTVWFELV(1)
SSTLYLIFE(1)
YFWLWDHHH(1)
WFLLWEPHH(1)
GLWGWEGFR(1)
TPGGFRGEG(1)
RVYLWIGEH(1)
EMELFWTHH(1)
ECVVWGRHH(1)
VAALWALFH(1)
QGGKWALAY(1)
ATYRWAASG(1)
GPVVFLVRY(1)
VLGIFLAVR(1)
GSGQFLLFL(1)
WAVWFLLIP(1)
GYVTFLLIM(1)
GWSTYLSYS(1)
GAGFSTGW(1)
VLYWFSSSW(1)
LGITFMILH(1)
LLWGFMFHH(1)
VLVWWAKRA(1)
GGNWWGVAT(1)
GGLWWSYRS(1)
TPGGWCQVQ(1)
PGGDFRPAV(1)
TPGGFRIVT(1)
GGWVFRSLG(1)
LFIFWHAVH(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 30521603

Target Name:

Mast cell protease 1

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

pComb M13 phage display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

An aliquot of the amplified phages (~e9 pfu) were bound to 100 μl Ni-NTA beads by their His6-tags for 1 hr at 4°C under gentle agitation. Unbound phages were removed by washing ten times in 1.5 ml 1 M NaCl, 0.1% Tween-20 in PBS, pH 7.2, and two subsequent washes with 1.5 ml PBS. The beads were finally resuspended in 1 ml PBS. Activated mast cell protease 1 (~0.1 μg) was added to the resuspended beads and left to digest susceptible phage nonapeptides under gentle agitation at room temperature for 2 hours. PBS without protease was used as control. Phages with a random peptide that was susceptible to protease cleavage were released from the Ni-NTA matrix, and the supernatant containing these phages was recovered. To ensure that all of the released phages were recovered the beads were resuspended in 100 μl PBS (pH 7.2) and the supernatant, after mixing and centrifugation, was added to the first supernatant. To ensure that the His6-tags had been hydrolyzed on all phages recovered after protease digestion, 15 μl fresh Ni-NTA agarose beads were added to the combined phage supernatant and the mixture agitated for 15 min followed by centrifugation.

BiopanningDataSet Comments:

Curator:

Zhongjie Tang

Curation Date:

2019-01-29

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