BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3369

Peptides:

Sequences

GCAFVMKP(1)
GCPYMIRP(1)
GCAYVLRP(1)
GCTLVFRP(1)
GCAFELRP(1)
GCSFVLKP(1)
GCNFKLSP(1)
GCTLMYRP(1)
GCPLILKP(1)
GCSFIMRP(1)
GCAFTLRP(1)
GCPYKLSW(1)
GCPFLLRF(1)
GCTYLHRP(1)
GCSWIYKP(1)
GCTWLYRP(1)
GCPFALRL(1)
GCSFQLRQ(1)
GCLFQLRP(1)
GCSFELRP(1)
GCSWVLSP(1)
GCSFILRP(1)
GCTYLYRP(1)
GCTFVLKP(1)
GCNFMFRP(1)
GCAYLLRP(1)
GCHFILKP(1)
GCTFILRP(1)
GCTFVYSP(1)
GCSWQLRP(1)
GCTFLLRP(1)
GCVLSLRP(1)
GCTFYLKP(1)
GCVYIFRP(1)
GCTMILKP(1)
GCTLQLRP(1)
GCAMILRP(1)
GCGWILRP(1)
GCSWVLRP(1)
GCAYTLRP(1)
GCTMVLRP(1)
GCNYILKP(1)
GCQWSLRP(1)
GCTMEWRP(1)
GCSMIFKP(1)
GCTFVLRP(1)
GCFYLLRP(1)
GCPFILKL(1)
GCPLIFKP(1)
GCTYILRP(1)
GCSFLLRP(1)
GCPYVLKP(1)
GCALMLRP(1)
GCAFVIRP(1)
GCTFSLRA(1)
GCAFSLRR(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 30543823

Target Name:

Chymotrypsin

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

SPINK1 GCX6 M13 phage display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Briefly, chymotrypsin was immobilized on MaxiSorp (Nunc) plates. Phage particles were obtained with the PEG/NaCl precipitation method and applied to the blocked wells. Following thorough washing, bound phages were eluted. E. coli XL1-Blue was used to amplify the eluted phages to be used in a next round of selection. After three selection and amplification cycles, the libraries were enriched over 100-fold compared to the control obtained on blocked wells containing no target enzymes.

BiopanningDataSet Comments:

Curator:

Lixu Jiang

Curation Date:

2019-01-29

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