BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3364

Peptides:

Sequences
SSRLMIL AAWLSPL GSFLYHP SFTHLLY LTPVYYW SSHMPHW

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (competitive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 11397457

Target Name:

Lipase (EC:3.1.1.3)

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Biopanning was performed against lipase attached to magnetic beads. Bound phage were eluted by resuspending the beads in 100 μl of 100 μg/ml lipase solution in TBST, incubated at room temperature for 1 h.

BiopanningDataSet Comments:

The synthetic heptapeptide GSFLYHP did not act as a satisfactory affinity ligand after immobilization on Sepharose. This indicated that the interaction with lipase was due to both the heptapeptide and the presence of a part of the phage coat protein. This conclusion was further verified by immobilizing the whole phage on the surface of magnetic beads and using the resulting conjugate as an affinity adsorbent.

Curator:

Bifang He

Curation Date:

2019-01-29

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