BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3346

Peptides:

Sequences
CTFAGSSC(91%) CNSAFAGC(3%) CFPKRVTC(3%) CPRSAKNC(3%)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 25675522

Target Name:

Purified serum IgG from a year 7 patient with advancing prostate cancer

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

CX6C phage display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

In brief, a CX6C (C, cysteine; X, any residue) phage-displayed peptide library was precleared on a pool (50 samples) of protein G-purified antibodies from age-matched healthy donor men to remove nonspecific peptides. Next, the precleared library was incubated with purified serum IgG from a prostate cancer patient of year 7. Three rounds of selection were performed.

BiopanningDataSet Comments:

We evaluated binding of the isolated serum antibodies from the different time points to the CTFAGSSC peptide and found an increase in reactivity against the peptide over time (year 0, year 1, year 5 and year 7), with the sample from year 7 producing the most robust binding. Binding of year 7 antibodies to CTFAGSSC could be inhibited in a concentration-dependent manner with preincubation of the synthetic peptide CTFAGSSC, whereas preincubation with a control peptide, CKDRFERC, did not inhibit binding. Immunostaining with autologous purified antibodies on prostate cancer biopsies from the index patient showed reactivity that could be inhibited by preincubation with the synthetic peptide CTFAGSSC. These findings confirm the specificity of the isolated antibodies for the peptide CTFAGSSC. We further identified alpha-2–Heremans–Schmid glycoprotein (AHSG, also known as fetuin-A) as the putative protein corresponding to the peptide mimic.

Curator:

Ying Wang

Curation Date:

2018-10-20

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