BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3342

Peptides:

Sequences
INHQLDTTQILV(5) FPLETSHMSAPL(4) YSSALKTLPIFQ(2) KVFEQDLLTTIL(2) SMQLMTSRLTWN(1) NILTTTWLPLHG(1) TELTTHPVFQFR(1) YQGDANLKTWHV(1) NLVTKPLHVSHL(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 29990379

Target Name:

CD177 antigen

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

To eliminate peptides that bind to Chinese hamster ovary (CHO) cells, 1e9 phage in PBS containing calcium and magnesium (PBS++), and supplemented with 0.5% bovine serum albumin (BSA), were incubated three rounds with wild-type CHO cells plated on 10 cm tissue culture plates, each for 1 h at room temperature on a rocker. Unbound phage were then incubated with CHO cells expressing human CD177.

BiopanningDataSet Comments:

Curator:

Yuwei Zhou

Curation Date:

2018-10-20

First |

Prev |

3342

| Next

| Last