BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3329

Peptides:

Sequences

Affinity Values

CNTGSPYEC(2.53%)
CLKLGEKWC(1.10%)
CSISSLTHC(0.88%)
CMARYMSAC(0.77%)
CLMTSQFRC(0.55%)
CRGATPMSC(0.66%)
CTVRTSADC
CNWGDRILC
CGYSSFNRC
CSHMEYPRC

[1.09]
[0.87]
[2.50]
[2.12]
[0.73]
[0.70]
[2.47]
[0.78]
[NT]
[NT]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Illumina HiSeq™ 2000

Sequencing Method:

Next generation sequencing

Rounds of Panning:

Reference:

PMID: 29777796

Target Name:

Human breast cancer cell line MDA-MB-231

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-C7C phage display library (CX7C)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

The affinity valune were read using an ELISA plate reader at 450 nm.

Brief Description:

First, MDA-MB-231 cells were prepared as a 1e6 cell suspension in 500 μl of DMEM containing 5% bull serum albumin and were incubated with 1e11 pfu of ph. D.-CX7C phage library on ice for 2 h. Thereafter, unbound phages were washed away. Specific-binding phages were transferred into the organic lower phase and were amplified with coliER2738 host cells. After three rounds, the phages were titrated on plates and were picked up for DNA extraction.

BiopanningDataSet Comments:

As the binding activity of the S3 phage was positively correlated to the level of NKA α1 expression in various breast cancer cells, NKA α1 was validated as the primary target of the S3 peptide.

Curator:

Ning Li

Curation Date:

2018-07-23

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