BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3327

Peptides:

Sequences	Affinity Values
YDMPNTV YDMHNTV YDLHNTV YDMHNAV NERALTL FLSDTRS GSGNAIM GSGNAHR	[NT] [NT] [NT] [NT] [NT] [0.91] [1.16] [NT]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

BigDye terminator v3.1

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 29846456

Target Name:

Anti-Haemonchus contortus polyclonal antibody

Template Name:

Haemonchus contortus

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

The optical density was determined using a microplate reader (BioTek, USA) at 405 nm at 28 days.

Brief Description:

Te aim of this study was to evaluate phage display technology for mapping Haemonchus contortus mimotopes. We screened the PhD 7 Phage Display Peptide Library Kit with a sheep polyclonal antibody against H. contortus. After four rounds of selection, 50 phage peptide clones were selected by biopanning and sequenced.

BiopanningDataSet Comments:

Curator:

Ning Li

Curation Date:

2018-07-23

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