BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3310

Peptides:

Sequences
LGDLHNRDNNSA(1) HSRTDYVQASYP(1) GLHTSATNLYLLH(5) GDGNSVLKPGNW(1) TASDVPRSRPHS(1) SGVYKVAYDWQH(5)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (in vivo)

Sequencing Platform:

Eurofins

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 29473787

Target Name:

Mouse heart

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

A total of three pannings were performed. Briefly, at day 21 after induction of EAM, animals received an intravenous injection through the tail vein with either a linear 12 amino acid (AA) peptide phage library or an empty phage at a concentration of 1e9 plaque-forming units (pfu)/μl of PBS.For each panning, animals were divided into the following treatment groups: MyH PhD, MyH Cont,PBS PhD and PBS Cont. The hearts for each of the four treatment groups were weighed, pooled together and homogenized. The resulting homogenate solution for each treatment group was then used to amplify, purify and titer the phages that bound to the hearts in each treatment condition. The phages isolated in panning 1 were injected at 1 × 109 pfu/μl of PBS (110 μl total volume) to animals in the same treatment group in panning 2, and the phages isolated in panning 2 were injected at same concentration and volume to animals in the same treatment group in panning 3.

BiopanningDataSet Comments:

We identified one peptide, MyH-PhD-05, that targets severe myocarditis in vivo.

Curator:

Ning Li

Curation Date:

2018-04-19

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