BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3299

Peptides:

Sequences

Affinity Values

PGATAGPSS
STRRIGWLTD
TDQTHRLA
PGATAGPSS
PGATAGPSS
TDQTHRLA
STRRIGWLTD
SWVHEWVTSN
TDQASRLA
RSGCTSGLHR
TDQASRLA
RSGCTSGLHR

[1.47 ± 0.04]
[1.50 ± 0.03]
[1.46 ± 0.05]
[1.55 ± 0.04]
[1.38 ± 0.04]
[1.42 ± 0.07]
[1.46 ± 0.03]
[1.56 ± 0.01]
[1.46 ± 0.03]
[1.51 ± 0.04]
[1.45 ± 0.03]
[1.43 ± 0.03]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 29522802

Target Name:

Anti-S.aureus GapC monoclonal antibody(mAb2A9)

Template Name:

Glyceraldehyde-3-phosphate dehydrogenase

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Detection of the binding activities of 12 strongly positive phage clones to mAb2A9 that were selected by a sandwich ELISA and the OD450 value of each well was read using a microplate reader (Bio-Rad, Hercules, CA, USA). Wild-type M13 phage was used as the negative control and an E. coli ER2738 culture supernatant was used as the blank control. Bovine serum albumen-coated wells were used to exclude cross-activity.

Brief Description:

BiopanningDataSet Comments:

The consensus motif PVATGSLT of the 12 clones is located at amino acids 236–243 of the GapC protein. These results suggest that the motif 236PVATGSLT243 is an epitope of the S. aureus GapC protein and it might be a potential component of an epitope-based vaccine against S. aureus.

Curator:

Lixu Jiang

Curation Date:

2018-04-19

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