BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3296

Peptides:

Sequences
CSYKARVTC

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 29516383

Target Name:

EAE-CNS brain endothelial cell

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-C7C phage display library (CX7C)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

CNS cells were prepared from rat CNS. A rat with acute EAE (score 3) was deeply anesthetized and perfused with 50 mL ice-cold PBS 1×. The whole CNS tissues from both rats were recovered separately (about 2.3 g), cut into small pieces, and incubated for 50 min at 37 °C in 10 mL of PBS 1×. The recovered cells were concentrated and frozen. The Bex vivo total EAE selection^ was performed in two steps: (i) a counter-selection with CNS cells from a healthy rat realized to absorb healthy tissuespecific phages and (ii) the selection against cells from the EAE CNS. Thus, 1011 phages were incubated with 4.0 × 106 CNS cells from a healthy rat before incubation of the unbound phages with CNS cells from a EAE rat (4.0 × 106). After incubation, the cells were pelleted by centrifugation (1200 rpm/5 min/RT) and washed with 1 mL complemented RMPI. The phages that remained associated with cells were amplified. At each selection round, one additional washing step of cells associated with phages was added to increase the selection stringency, so that five washes were performed during the fifth round.

BiopanningDataSet Comments:

This peptide mimics the discointeracting protein 2 homolog A (DIP2A), receptor for Follistatin 1, a glycoprotein involved in inflammatory reactions (Ph36).

Curator:

Lixu Jiang

Curation Date:

2018-04-19

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