BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3293

Peptides:

Sequences	Affinity Values
ATRSANGM EQVPYPGE ASRSPTDK ASRGTIAG	[6.18 ± 0.48] [0.29 ± 0.07] [0.27] [3.09]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 29414074

Target Name:

Prostate-specific antigen

Template Name:

Anti-human total PSA monoclonal antibody

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

f8-8mer phage display library (X8)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

The phage was loaded into wells of a 96-well microtiter plate, and 50 μL of t-PSA solution was added to the wells, and the plate was incubated at 37 °C. Then the wells were washed six times with PBST, anti-PSA mAb was added. The plate was washed again, and then 50 μL of IgG-HRP was added and the plate was incubated for 1 h at room temperature. For the detection of t-PSA, 100 μL of 0.04% OPD was added. The absorbance at 492 nm was recorded on a microplate reader.

Brief Description:

~10e11 phage virions from f8/8 landscape phage library in 50 μL library diluents were added to the wells as the input for the first round of biopanning. After incubation overnight at 4 °C, the wells were washed six times with TBST to remove unbound phages. After three rounds of selection, individual phage clones were picked randomly and amplified.

BiopanningDataSet Comments:

Peptide ATRSANGM showed highest affinity and specificity against t-PSA.

Curator:

Lixu Jiang

Curation Date:

2018-04-19

First |

Prev |

3293

| Next

| Last