BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3288

Peptides:

Sequences	Affinity Values
VHWDFRQWWQPS(5) GDGNSVLKPGNW(1) HLSLPLWKWEKS(1) VFAYHLRIPSGD(1)	[1.04±0.08] [0.49±0.07] [0.61±0.09] [0.43±0.13]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 29163684

Target Name:

The second extracellular loop (107-130) of C-C chemokine receptor type 9

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Affinity was detected using ELISA. The OD450 values were reproduced from Figure 1 in the literature.

Brief Description:

BiopanningDataSet Comments:

P1 was (VHWDFRQWWQPS) able to bind specifically with MOLT4 cells which exhibit marked expression of CCR9. In addition, P1 promoted the apoptosis of MOLT4 cells induced by doxorubicin, and inhibited the migration of MOLT4 cells in the presence of chemokine (C-C motif) ligand 25. It was suggested that decreased activity in the phosphorylation of protein kinase B in MOLT4 cells may be responsible for the inhibition. In conclusion, the peptide P1 derived from a screened phage is able to specifically bind to CCR9 and inhibit the activity of CCR9. It has potential use as an antagonist in the treatment of CCR9-overexpressed carcinoma.

Curator:

Zhongjie Tang

Curation Date:

2018-01-19

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