BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3286

Peptides:

Sequences	Affinity Values
YKRGYK(50) WPQGPT(11) ETWGYK(6) TRSGPT(3) RNSGWS(3) ARAGTT(2)	[10 ± 2, 620 ± 60] [NT, NT] [NT, NT] [NT, NT] [NT, NT] [NT, NT]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Applied Biosystems 3130 Capillary Sequencer

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 29050803

Target Name:

Platinum

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

T7 LARFH gene-based phage display library (X3GX2)

Affinity Measurement Method:

Quartz crystal microbalance (QCM)

Affinity Measurement Description:

The interaction of YKRGYK with a platinum surface was monitored and quantiﬁed by usinga hand-made flow-quartz crystal microbalance system (f-QCM) with a custom-made acryl ﬂow cell equipped with 9-MHz Pt or Au quartz crystals (Kyocera, Japan). The dissociation constant (KD, nM) was shown.

Brief Description:

BiopanningDataSet Comments:

The majority of the selected variants contained the Tyr-Lys-Arg-Gly-Tyr-Lys (YKRGYK) sequence in their randomized segment. We characterized the platinum-binding properties of mutant LARFH by using quartz crystal microbalance analysis. Mutant LARFH seemed to interact with platinum through its loop containing the YKRGYK sequence, as judged by the estimated exclusive area occupied by a single molecule. Furthermore, a 10-residue peptide containing the YKRGYK sequence bound to platinum with reasonably high afﬁnity and basic side chains in the peptide were crucial in mediating this interaction. In conclusion, we have identiﬁed an amino acid sequence, YKRGYK, in the loop of a helix-loop-helix motif that shows high platinum-binding afﬁnity. This sequence could be grafted into loops of other polypeptides as an approach to immobilize proteins on platinum electrodes for use as biosensors among other applications.

Curator:

Zhongjie Tang

Curation Date:

2018-01-19

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