BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3280

Peptides:

Sequences

CSPGAKVRC(322/143637)
CGEKRTRGC(279/143637)
CDSRSRKRC(226/143637)
CPKGAGSKC(196/143637)
CRKGTLGRC(149/143637)
CVKGTVKTC(148/143637)
CRGTIRGRC(143/143637)
CLDRRKKPC(136/143637)
CPARRSNRC(117/143637)
CKDSAEMRC(113/143637)
CERSNNVSC(113/143637)
CGKRAPFRC(110/143637)
CVSSSSGRC(108/143637)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (in vivo)

Sequencing Platform:

Ion Torrent semiconductor sequencing system

Sequencing Method:

Next generation sequencing

Rounds of Panning:

Reference:

PMID: 29116108

Target Name:

Peritoneal macrophage of mouse bearing breast cell line 4T1

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

T7 CX7C phage display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Naïve phage library was injected intraperitoneally in 4T1 tumor-bearing mice, and allowed to circulate for 2 h. Peritoneal cells were collected, the accompanying phages were rescued and the peptide-encoding segment of phage DNA was sequenced.

BiopanningDataSet Comments:

Tumor-associated macrophages (TAMs) expressing the multi-ligand endocytic receptor mannose receptor (CD206/MRC1) contribute to tumor immunosuppression, angiogenesis, metastasis, and relapse. Here, we describe a peptide that selectively targets MRC1-expressing TAMs (MEMs). Deep sequencing of the peptide-encoding inserts in the selected phage pool revealed enrichment of the peptide CSPGAKVRC (codenamed “UNO”). Intravenously injected FAM-labeled UNO (FAM-UNO) homed to tumor and sentinel lymph node MEMs in different cancer models: 4T1 and MCF-7 breast carcinoma, B16F10 melanoma, WT-GBM glioma and MKN45-P gastric carcinoma. Fluorescence anisotropy assay showed that FAM-UNO interacts with recombinant CD206 when subjected to reducing conditions. Interestingly, the GSPGAK motif is present in all CD206-binding collagens. FAM-UNO was able to transport drug-loaded nanoparticles into MEMs, whereas particles without the peptide were not taken up by MEMs. In ex vivo organ imaging, FAM-UNO showed significantly higher accumulation in sentinel lymph nodes than a control peptide. This study suggests applications for UNO peptide in diagnostic imaging and therapeutic targeting of MEMs in solid tumors.

Curator:

Ning Li

Curation Date:

2018-01-19

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