BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3266

Peptides:

Sequences	Affinity Values
HLWGWLYAPSF(15) HYSWSWIAYSPG(2) VHWDFRQWWQPS(2) YSWHIDIVAPRN(2) YHHSGPYAGPMW(1) SVYVEISWVRTM(1) DWSSWVYRDPQT(1)	[0.95] [1.14] [1.47] [Infinite ratio] [2.50] [1.17] [2.36]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Beckman Coulter Genomics

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 28976753

Target Name:

Galectin-1, Gal-1

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Peptide P7 (YSWHIDIVAPRN) was selected according to its affinity toward gal-1. Its binding has been revealed by immunohistochemistry on human thyroid cancer biopsies, and it was co-localized with gal-1 by immunofluorescence on TPC-1 cell line. Peptide P7 induced a decrease in TPC-1 cells' adhesion to gal-1 immobilized on culture plates. After coupling to USPIO, the peptide preserved its affinity toward gal-1. Its specific binding has been corroborated by co-localization with gal-1 expressed by TPC-1 cells and by its ability to compete with anti-gal-1 antibody. The peptide and its USPIO derivatives produce no toxicity in HepaRG cells as determined by MTT assay. The vectorized contrast agent is a potential imaging probe for thyroid cancer diagnosis. Moreover, the gal-1-targeted peptide prevent cancer cell adhesion by interacting with the carbohydrate-recognition domain of gal-1.

Brief Description:

A linear 12-mer random peptide phage display library (PhD-12, New England Biolabs Inc. Bioké, Leiden, The Netherlands) was screened against human gal-1 (Creative Biomart, New York, USA) for three rounds, which was immobilized on a 96-well ELISA plate at a concentration of 100 µg/mL. The preselection step was performed by incubating the phage library with a mix of human serum albumin (HSA) and collagen (both at 100 µg/mL; Sigma-Aldrich). The time for the preselection step is modified along the rounds (80 – 100 – 120 min).

BiopanningDataSet Comments:

Curator:

Lixu Jiang

Curation Date:

2018-01-19

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