BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3256

Peptides:

Sequences
YYWGPPV(2) HYIDFRW(2) WNEVKPI(1) IIIRNSF(1) NYTLTDI(1) AQNQPMA(1) SLLGQTP(1) GLFHNAQ(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 28888997

Target Name:

Human prostate cancer cell line PC3

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

PC-3 cells were seeded onto a well of a 96 well microplate (Nunc Maxisorb) for 24 hours to bring the culture to about 75% confluence. The cells were washed with PBS and then exposed to 1.0e9 phage particles from the parent Ph.D-7 library in 100µl PBS. After incubation, the cells were washed with PBS and then 100µl of 0.2M Glycine-HCl, pH. 2.2 were added to elute cell-bound phage. After 10 mins, 15µl of 1M Tris-HCl, pH 9.1 was added to neutralize the solution and the eluted phage were recovered. The cells were washed again with PBS and then 100µl 30mM Tris, pH. 8.0 was added. The cells were frozenthawed until they had ruptured as judged by light microscopy (three cycles). The ruptured cell mixture was collected and centrifuged at 2000rpm for 2 min; the supernatant containing the PC3 internalized phage was recovered. Phage were titrated, amplified and the exposure procedure was repeated twice more.

BiopanningDataSet Comments:

Curator:

Pengcheng Yao

Curation Date:

2017-10-24

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