BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3253

Peptides:

Sequences
NKLLATW(18) NKLCCTK(1) NKLCCAK(1) GQLLCTM(1) GQLLATK(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 28853252

Target Name:

Anti-VP2 monoclonal antibody 3G11

Template Name:

Outer capsid protein VP2

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

The results of sandwich ELISA test shown that MAb 3G11 can react positively with BTV-8, and with other 23,no positive reaction was found in the virus species.

Brief Description:

BiopanningDataSet Comments:

After the fourth round of panning, obtained positive sequence clones KLLAT, the 3 amino acids LL and T of the sequence are consistent with the amino acid residue LLST sequence of BTV-8 VP2.

Curator:

Pengcheng Wu

Curation Date:

2017-10-24

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