BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3248

Peptides:

Sequences

CPFMSVANC(2)
CAISSPGSC(1)
CNAKHHPRC(1)
CDSRWHSVC(1)
CNGGYAFEC(1)
CEHHKNTLC(1)
CNSRLNQHC(1)
CEIWSRAAC(1)
CPGEPPTLC(1)
CELQGWHLC(1)
CPMFLASLC(1)
CEPSLIPFC(1)
CPRVNMLHC(1)
CGENIQQYC(1)
CQNHQSRHC(1)
CGLNKTPGC(1)
CRSDSYNKC(1)
CGPKTPHKC(1)
CSGALTSPC(1)
CGSKLDALC(1)
CSKQSTIAC(1)
CHQWNSNSC(1)
CSMEELVTC(1)
CHTAHKATC(1)
CSTEHTRSC(1)
CKDQGQMRC(1)
CTASPSGLC(1)
CKPHTGHVC(1)
CTDPNSTTC(1)
CKPPGSYFC(1)
CTNANSHLC(1)
CLDSPNNVC(1)
CTPDASRKC(1)
CLKLTAASC(1)
CTSNTKFNC(1)
CLNDSVHQC(1)
CTTYNATSC(1)
CLNVPTKS(1)
CWPPSTYSC(1)
CLSDYSIMC(1)
CYGKNNQRC(1)
CMLNTSVAC(1)
CYSELTGNC(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 28757359

Target Name:

The human colon carcinoma Caco-2 cell

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-C7C phage display library (CX7C)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

BiopanningDataSet Comments:

Phage displaying cyclic heptapeptide DNPGNET (DNP-phage) showed the greatest permeability across a Caco-2 cell monolayer and mouse intestinal epithelium. Interestingly, DNPGNET (DNP) does not contain any basic amino acids. Its isoelectric point (pI) was estimated to be 2.72. It did not reduce the viability or tight-junction integrity of Caco-2 cells at concentrations up to 100 μM for 24h. Up take of either DNP phage or fluorescence-labeled DNP derivative (AC-DNPGNET-CGGGS modified with 5/6-FAM at the C-terminal; the cysteines serve to generate the cyclic peptide via disulfide bond formation, and GGGS is the phage linker) by Caco-2 cells was inhibited by low temperature, unlabeled AC-DNPGNET-CGGGS and EIPA, a macropinocytosis inhibitor. Thus, DNP appears to facilitate transcellular permeability of phages via macropinocytosis, but not paracellular diffusion. These findings indicate that DNP is a promising candidate as a carrier to promote intestinal absorption of macromolecular drugs.

Curator:

Yewei Fang

Curation Date:

2017-10-24

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