BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3239

Peptides:

Sequences	Affinity Values
QVNGMLGERSQQ(5) VYPYPKVDLSQD(3) DVSSLYDGPVGD(2) HAQGSLGTPVFM(2)	[0.77, 0.21] [0.64, 0.28] [0.64, 0.27] [0.83, 0.24]

Sequences Features:

Motif:

Y-x(2)-P-x-G-D-L-G

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 28711695

Target Name:

Anti-FMDV monoclonal antibody 6C9

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Four phage clones were selected after three rounds of biopanning, and their binding activity to MAb 6C9 was analyzed in phage ELISA. Murine MAb 5D12 and wild-type M13 phage (PL) were used as negative controls. For each clone, the absorbance at 450 nm was calculated as an average of three duplicates. Data shown were absorbance values (the first column of the affinity values) reproduced from Figure 2. Additionally, binding inhibition of phage clones and whole virus antigens to MAb 6C9 were analyzed in competitive ELISA. TBS was used as a negative control. For each clone, the absorbance at 450 nm was calculated as the average of three duplicates. Data shown were absorbance values (the second column of the affinity values) reproduced from Figure 3.

Brief Description:

The M13 phage display library displaying 12-mer random peptides (Ph.D-12 Phage Display Peptide Library Kit, New England Biolabs) was used. Affinity selection of the phage clones from the random peptide library was conducted following the manufacturer's recommendations with minor modifications.

BiopanningDataSet Comments:

Screening of a phage-displayed random 12-mer peptide library revealed that MAb 6C9 bound to phages displaying the consensus motif YxxPxGDLG.

Curator:

Ning Li

Curation Date:

2017-10-24

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