BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3237

Peptides:

Sequences
DWYRDPVFDVLL LWRRVVVDSYGV YMWIACPDGWDC WWDWAVQSETLL WWVNPLGVTQDV EYPWWMEQFYLE WWAEAWFAWTPG ISWHSDWWVELV

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 28701344

Target Name:

The μ (mu) homology domain of suppressor of yeast profilin deletion

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

BiopanningDataSet Comments:

From the screen, we identified eight potential Syp1 ligand sequences and, to aid in the search for potential motifs, we compared the peptide sequences to that of Mid2 (251-316), the smallest fragment of Mid2 previously shown to bind the Syp1 μHD. Two DxY sequences (DWYRDPVFDVLL and LWRRVVVDSYGV), two YxxΦ motifs (YMWIACPDGWDC and EYPWWMEQFYLE), and several instances of two consecutive residues with bulky side chains, including one WY (DWYRDPVFDVLL) and five WW sequences (WWDWAVQSETLL, WWVNPLGVTQDV, EYPWWMEQFYLE, WWAEAWFAWTPG and ISWHSDWWVELV), were present in the peptides. All of these potential Syp1-interacting motifs are present in Mid2 (251-316).

Curator:

Ning Li

Curation Date:

2017-10-24

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