BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3224

Peptides:

Sequences
KCCFPAQ(13) SILPYPY(4) YPAPWPP(3) QPWPTSI(3) WPTPPYA(1) MHAPPFY(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

ABI Automatic DNA Analyzer

Sequencing Method:

Sanger

Rounds of Panning:

Reference:

PMID: 28012848

Target Name:

Human colorectal cancer cell line HT29

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Three rounds of biopanning with a total of ~1e9 plaque-forming units (pfu) was performed with ~1e6 human normal intestinal cells Hs738.St/Int (control) to remove non-specific binders. The supernatant containing the cleared phage pool was amplified to ~2e11 pfu for positive selection against HT29 cells.

BiopanningDataSet Comments:

We identified the peptide sequence KCCFPAQ, and measured an apparent dissociation constant of kd = 72 nM and an apparent association time constant of k = 0.174 min–1 (5.76 min). During fluorescence imaging of patients during endoscopy, regions of SSA had 2.43-fold higher mean fluorescence intensity than that for normal colonic mucosa. Fluorescence labeling distinguished SSAs from normal colonic mucosa with 89% sensitivity and 92% specificity. The peptide had no observed toxic effects in animals or patients. In the analysis of ex vivo specimens, peptide bound to SSAs had significantly higher mean fluorescence intensity than to hyperplastic polyps.

Curator:

Ning Li

Curation Date:

2017-07-22

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