BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3222

Peptides:

Sequences

GAMHLPWHMGTL(3/13)
GAMHLSWHMGTH(1/13)
DPMHNNWHSSPI(1/13)
GLDHLWWSSQTP(1/13)
NPWEEQGYRYSM(1/13)
NPWNEMWFQTSR(1/13)
NNPWREMMYIEI(1/13)
WADMMTSVTPWL(1/13)
SEFPRSWDMETN(1/13)
QHYETLAFRPKH(1/13)
ATYNSVNRHSAV(1/13)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger

Rounds of Panning:

4/5

Reference:

PMID: 28529640

Target Name:

Huamn large cell carcinoma (LCC) cell line H460

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

BiopanningDataSet Comments:

Three targeting phages (HPC1, HPC2, and HPC4) and their respective displayed peptides (GAMHLPWHMGTL, NPWEEQGYRYSM and NNPWREMMYIEI) were able to bind to both SCLC and NSCLC cell lines, as well as clinical specimens, but not to normal pneumonic tissues. In vivo optical imaging of phage homing and magnetic resonance imaging (MRI) of peptide-SPIONs revealed that HSP1 was the most favorable probe for multimodal molecular imaging. Using HSP1-SPION, the T2-weighted MR signal of H460 xenografts was decreased up to 42%. In contrast to the tight binding of HSP1 to cancer cell surfaces, NNPWREMMYIEI was preferentially endocytosed and intracellular drug delivery was thereby effected, significantly improving the therapeutic index of liposomal drug in vivo. Liposomal doxorubicin (LD) conjugated to GAMHLPWHMGTL, NPWEEQGYRYSM or NNPWREMMYIEI had significantly greater therapeutic efficacy than non-targeting liposomal drugs in NSCLC (H460 and H1993) animal models. Combined therapy with an NNPWREMMYIEI-conjugated stable formulation of liposomal vinorelbine (sLV) further improved median overall survival (131 vs. 84 days; P = 0.0248), even in aggressive A549 orthotopic models. Overall, these peptides have the potential to guide a wide variety of tailored theranostic agents for targeting therapeutics, non-invasive imaging, or clinical detection of SCLC and NSCLC.

Curator:

Ning Li

Curation Date:

2017-07-22

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