BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3204

Peptides:

Sequences	Affinity Values
GLHTSATNLYLH(7/10) AGYPLSENFYYP(1/10) WQDFGAVRSTRS(1/10) FHPRLQQDHWLH(1/10)	[0.38 ± 0.28] [4.08 ± 0.31] [3.15 ± 0.36] [3.92 ± 0.44]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (competitive panning)

Sequencing Platform:

Sequencing Method:

Sanger

Rounds of Panning:

Reference:

PMID: 28094103

Target Name:

Particulate matter, PM

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Binding assay

Affinity Measurement Description:

Spot intensity measurements (with background subtraction) using Image Quant. The data shown were reproduced from the Figure 1B in the reference.

Brief Description:

Bound phages were eluted using a PM suspension [100 μg PM/ml TBS (50 mM Tris-HCl (pH 7.5), 150 mM NaCl)].

BiopanningDataSet Comments:

Four PM binding peptides were obtained by phage display and binding characteristics of these peptides were investigated using the peptide array. The strongest binding peptide, WQDFGAVRSTRS, displayed a binding property, measured in terms of spot intensity, 11.4 times higher than that of the negative control, AAAAA. Inductively coupled plasma mass spectrometry (ICPMS) analysis of the transition metal compounds in the PM bound to the peptide spots was performed, and two peptides showed higher binding towards Cu and Zn compounds in PM. These results suggest that the screened peptides could serve as an indicator of transition metal compounds, which are related to adverse health effects, contained in PM.

Curator:

Juanjuan Kang

Curation Date:

2017-07-22

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