BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3200

Peptides:

Sequences

YPLRPNAESLRF
DPIYALSWSGMA
NATHLTADHVNK
YPSAPPQWLTNT
AQHIRSWDGFSH
LPLYTSPDKPGK
SILNYPTNPGIA
LLADTTHHRPWT
HPIRVQPDWGFL
VETHTTQWLITEV
TPMGRPHPETPA
SGKTSLISHAAL
NHLPLPPPAATM
SDTQMPPAXGRA
GYLPLHSITYRP
QLLEPVNLSTGP
WHPPKGLSPLPD
FRLPGSLINHPQ
SILSTMSPHGAT
NYNPHNPFPPAP

Sequences Features:

Motif:

NHT, [ST]-[IV]-L-S

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger

Rounds of Panning:

Reference:

PMID: 28453953

Target Name:

Human bone marrow stromal cell (hBMSC)

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

The NEB 12-mer peptide library was prescreened against sintered Hydroxyapatite (HAP) disks prior to introduction to the cells.

BiopanningDataSet Comments:

A peptide sequence with high affinity towards apatite (VTKHLNQISQSY, VTK) was identified using phage display. In this study, a combinatorial phage display identified the cell binding sequence (DPIYALSWSGMA, DPI) which was combined with the mineral binding sequence to generate the dual peptide DPI-VTK. DPI-VTK demonstrated significantly greater binding affinity (1/KD) to apatite surfaces compared to VTK, phosphorylated VTK (VTKphos), DPIVTKphos, RGD-VTK, and peptide-free apatite surfaces (p < 0.01), while significantly increasing hBMSC adhesion strength (t50, p < 0.01). MSCs demonstrated significantly greater adhesion strength to DPI-VTK compared to other cell types, while attachment of MC3T3 pre-osteoblasts and murine fibroblasts was limited (p < 0.01). MSCs on DPI-VTK coated surfaces also demonstrated increased spreading compared to pre-osteoblasts and fibroblasts. MSCs cultured on DPI-VTK coated apatite films exhibited significantly greater proliferation compared to controls (p < 0.001). Moreover, early and late stage osteogenic differentiation markers were elevated on DPI-VTK coated apatite films compared to controls.

Curator:

Lixu Jiang

Curation Date:

2017-07-22

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