BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 32

Peptides:

Sequences	Affinity Values
VPWMEPAYQRPL TLGAQYLPTRTA MPPTDYCSLCIK PPAHNSKSPMKR MPYTNERNPSQP TPQLTTGIYPPR SLTMMSPPAASD LKMPVSELLLKK VPLPWHMYAPAR	[0.36] [0.17] [0.20] [0.29] [0.49] [0.58] [0.54] [0.38] [0.85]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 12527505

Target Name:

Osteosarcoma cell line OS-732

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Absorbance at 490 nm was dertemined. Results demonstrated that only 9 clones specifically bound to osteosarcoma cells. Data shown were reproduced from the graph.

Brief Description:

Osteoblasts were used as the absorber cells for subtraction biopanning from phage display library.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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