BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3198

Peptides:

Sequences	Affinity Values
CNHQHQKGC(3) CKSKNHPSC(2) CQSHKNNKC(1)	[108, 37.23] [21.0, 18.44] [N.A., N.A.]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger

Rounds of Panning:

Reference:

PMID: 28650615

Target Name:

Human Hair

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

ACX7CG phage display library

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Phage enzyme-linked immunosorbent assay (ELISA) experiments were performed in order to determine the apparent binding strength (Kapp, 1.0e10 M-1) and relative affinity (RAmax) of selected phage clones in comparison to the random phage library. The first column of the affinity value is Kapp, and the second RAmax.

Brief Description:

The hair samples were incubated with the shampoo formulations containing the phage library. The washing stringency was gradually enhanced by stepwise increasing the surfactant concentration from 0.1 w/w% (Round 1) to 0.4 w/w% (Round 4).

BiopanningDataSet Comments:

A peptide with the sequence of CNHQHQKGC that can bind to human hair under shampooing conditions was used to enhance the deposition of model fragrance delivery systems. These delivery systems are either based on poly(N-(2-hydroxypropyl)methacrylamide) (PHPMA) copolymers as a representative for polymeric profragrances or polyurethane/urea-type core-shell microcapsules as a model physical fragrance carrier. The incorporation of a hair binding peptide enhanced the deposition of PHPMA copolymers with a factor of 3.5-5.0 depending on the extent of peptide incorporation, whereas 10 w/w% surface functionalization of microcapsules with the peptide led to a 20-fold increase in their deposition. In a final experiment, treatment of the hair samples under realistic application conditions with the peptide- functionalized microcapsules resulted in an increase fragrance release from the hair surfaces.

Curator:

Lixu Jiang

Curation Date:

2017-07-22

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