BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3191

Peptides:

Sequences

Affinity Values

LSSTTSPRVTTS(6)
QQLPSSSTSTYP(5)
TVAHHYPPQTPL(5)
ITTGNEWSYSRK(4)
NKLPMPISVPLV(4)
TELGQVSNAIPW(3)

[0.889 ± 0.016]
[1.011 ± 0.031]
[0.832 ± 0.016]
[0.846 ± 0.054]
[0.995 ± 0.016]
[0.932 ± 0.021]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger

Rounds of Panning:

Reference:

PMID: 28634746

Target Name:

SiHa cells

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

The reaction was terminated by adding 2 M H2SO4 (50 ll/well) and measured at 450 nm by an ELISA reader.Irrelevant phage clone (IRP, an amplified phage clone randomly selected from the original phage-displayed peptide library) and PBS were used as negative controls. Data shown (OD450) were reproduced from Figure 1 in the reference.

Brief Description:

SiHa and HEK293 cells were used as positive target cells and negative absorber cells. Phages were incubated with HEK293 cells for 1 h after which the supernatant containing unbound phages was incubated with the blocked SiHa cells.

BiopanningDataSet Comments:

After sequencing of positive clones, six different peptide sequences were obtained and CSP3 (QQLPSSSTSTYP) showed best affinity and specificity to SiHa cells via immunofluorescence assay. Peptide CSP3 may be a potential candidate for molecular imaging detection and targeting therapy of cervical cancer.

Curator:

Lixu Jiang

Curation Date:

2017-07-22

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