BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3126

Peptides:

Sequences
KSLSRHDHIHHH(6) QVHINHISPSVP(2) KSLSRHDHLLPH(1) QSLSRHDPILHH(1) SFQAGIRSAPEP(1) SFQVGIRSAPEP(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger Sequencing

Rounds of Panning:

Reference:

PMID: 28272485

Target Name:

Anti-PRRSV monoclonal antibody A2

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

BiopanningDataSet Comments:

Anti-porcine reproductive and respiratory syndrome virus (PRRSV) neutralizing monoclonal antibody A2 recognize the mimotope (KSLSRHDHIHHH), P2, which contains the SRHDHIH motif, which has conserved consensus sequences from amino acid positions 156 to 161 in the N-terminal ectodomain of GP3. The artificial multi-epitope gene, mp2, was designed by combining three repeats of the mimotope P2. The resulting sequence was inserted into the swinepox virus (SPV) genome to construct a recombinant swinepox virus (rSPV-mp2). The rSPV-mp2 was able to stably express the multi-epitope peptide, mP2, in vitro. The rSPV-mp2 immunized pigs exhibited a significantly shorter fever duration compared with the wtSPV treated group (P < 0.05). There was an enhanced humoral and cellular immune response, decreased number of PRRSV genomic copies, and a significant reduction in the gross lung pathology (P < 0.05) was observed following PRRSV infection in rSPV-mp2-immunized animals.

Curator:

Bifang He

Curation Date:

2017-04-11

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