BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3122

Peptides:

Sequences
IQYRSWIPFSYP LDYRSWAPYATS YRSFDPWYPPVH LSIRSYTSPQWQ LNYRSYLPYETS

Sequences Features:

Motif:

YRSWxP

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

454 GS FLX Titanium Series Amplicon

Sequencing Method:

Deep sequencing

Rounds of Panning:

3/4

Reference:

PMID: 28035012

Target Name:

Viral protein 1 and 2 (VP1 and VP2)

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

BiopanningDataSet Comments:

After biopanning, phage populations were sequenced and analyzed to identify a consensus peptide motif—YRSWXP. Two 12-mer peptides containing this sequence, NV-O-R5-3 (LDYRSWAPYATS) and NV-O-R5-6 (IQYRSWIPFSYP), were further characterized to evaluate the motif’s functional ability to detect VLPs and virus. Results indicated that these peptides effectively detect GI.1 VLPs in solidphase peptide arrays, ELISAs and dot blots. Further, their specificity for the S-domain of the major capsid protein enables them to detect a wide range of GI and GII norovirus genotypes. Both peptides were able to detect virus in norovirus-positive clinical stool samples.

Curator:

KJJ

Curation Date:

2017-04-11

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