BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3118

Peptides:

Sequences	Affinity Values
*RGDDWA(20) RGDDWE(12) NRASNR(4) KKILH(4)**	[1.66955 ± 0.85] [1.10951 ± 0.85] [2.21998 ± 0.85] [NT]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger Sequencing

Rounds of Panning:

Reference:

PMID: 16787913

Target Name:

Kappa fractions of polyclonal immunoglobulin G(IgG) of multiplemyeloma patient MM077

Template Name:

Fibronectin(FN)

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

X6 T7 phage display library

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

The binding activities of phage clones to the Lactoferrin (LF) were measured by phage ELISA. The OD values at 450 nm were measured and data shown were reproduced from the Fig 1B in the reference.

Brief Description:

BiopanningDataSet Comments:

Two of the four peptides had the typical cell adhesion motif Arg-Gly-Asp (RGD), suggesting that human lactoferrin (hLF) may interact with RGD-containing proteins. Binding experiments using ELISA and surface plasmon resonance (SPR) analysis demonstrated that hLF bound to human fibronectin (hFN) and vitronectin (hVN) with relatively high affinity. Furthermore, the cell adhesion of Chinese hamster ovary (CHO), HeLa, and RAW cells to hFN or hVN coated on the culture plate was inhibited by hLF in a concentration-dependent manner, indicating that hLF interfered the cell adherent interaction through RGD motif.

Curator:

Yumin

Curation Date:

2017-04-11

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