BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3116

Peptides:

Sequences

NPVEDYLDYSVI
ESYYMNPVEMFV
LPTESNPVEDWI
NPIESYIASVFS
NPVELLLKTSSD
NPVETQIVLSLV
NPVEKWISMRTM
NPVELLLLMGIS
NGIERLLEEPVS
NPVENWIDPKSI
VNPVEYYLDTMR
NPVEALLSKFHM
NPVESYLANYTS
VTNPYESLVQEK
SPPEFSGSTVGL
MVPEFSGSFPMR
RMPPEFMGSLPQ
QLFVPEFAGSSP
LVPEFTGSTPFR
SHGAIEFDGAFP
VPEFAGHVPSTA
VPEMNGSLIARK
HPIVKTYFAQTT
APAKTYFGQTTD
QMKAWFPQTTYD
ATKVMFPQRIYV
QPAKTYFNQVTL
QAAAKTMFPQNT
SPSIDAFETSIF
SWVLTATETGSS
EMRFPSLSASDT
QAPTLDAQETAL
TALDAVSTGFSW
GAIGSLTADSTS
SLGSLSAYETGR
TLYRPPLTSAET
LIADLNAESTSR
SLGAAGARVTTV
ASGPLHAGATGL
GHKPVLTALSTA
VMPAIHAGVTGA
VHPTLTVTSKEI
ELDVSLRVMPKV
SKLAMEIMSGPV
SNAVTSSKSPRM
QADATVLTKPKT
KAVSDASRGTVF
DYPKIANFQEYA
GGQVRSIHSGPT
QHWPTNVDSVTV
ETKSDDMLLSNV
MTVDRTVRVASK
DLLIRDAITDTK
AYHVDTVSDAGW
VDTINLPQNTIQ
VMSVNASTTAAN
TLHAPMTIRSGP
TNLHRVMTVVNM
LRPNAVQTDTLA
QMRQLTEYGSEK
VLSSTAIKVDSV
VHTVHDVFTAFG
AKIRMFLDTDYK
ASWGPIAIDRVN
SQQYALTNSTTN
QPQTKSFYPQYV
NVVDRVNRTGVV
WSALPERTSLPV
RPAIVDQVSSSP
QWNWRVRSVANV
AQLHPTTLVKHK
TSYRPPLNVCQD
QSHSLFYPHPYG

Sequences Features:

Motif:

N-P-V-E-(x)3, x-P-E-F--G-S-x(2), K-x(2)-F-P-Q-x-T, L-x-A-x-E-T-x

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Illumina HiSeq1500

Sequencing Method:

Next generation sequencing

Rounds of Panning:

Reference:

PMID: 28167275

Target Name:

Serum antibodies

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

BiopanningDataSet Comments:

We synthesize identified peptides in the array format and rescreen the serum used for phage panning to validate antibody binding peptides. Finally, we systematically vary the sequence of validated antibody binding peptides to identify those amino acids within the peptides that are crucial for binding "their" antibody species. The resulting immune fingerprints can then be used to trace them back to potential antigens. We investigated the serum of an individual in this pipeline, which led to the identification of 73 antibody fingerprints. Some fingerprints could be traced back to their most likely antigen, for example the immunodominant capsid protein VP1 of enteroviruses, most likely elicited by the ubiquitous poliovirus vaccination.

Curator:

Yumin

Curation Date:

2017-04-11

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