BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3110

Peptides:

Sequences
WBRMPAYTAKYP WBRMPMETAKPL KNPTSTPAMYAN KPPQBTSAPYLP GBRNRANTSKNP GHWHSPHKLTRN

Sequences Features:

Motif:

RxPxxTxK

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger Sequencing

Rounds of Panning:

Reference:

PMID: 28220894

Target Name:

GST-N1-Src SH3 domain

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

BiopanningDataSet Comments:

By screening a peptide phage display library, we discovered a novel ligand (WHRMPAYTAKYP, PDN1) that targets the unique SH3 domain of N1-Src and inhibits N1-Src in cells. In cultured neurons, PDN1 fused to a fluorescent protein inhibited neurite outgrowth, an effect that was mimicked by shRNA targeting theN1-Src microexon. PDN1 also inhibited L1-CAM-dependent neurite elongation in cerebellar granuleneurons, a pathway previously shown to be disrupted in Src−/− mice. PDN1 therefore represents anovel tool for distinguishing the functions of N1-Src and C-Src in neurons and is a starting point for the development of a small molecule inhibitor of N1-Src.

Curator:

Curation Date:

0000-00-00

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