BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3101

Peptides:

Sequences
LPSAGRGVCYEA QHLNSILLVTK YPHGYVAFGRW

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (competitive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 27766741

Target Name:

Anti-LPS single-domain antibody fragment VHH

Template Name:

lipopolysaccharide, LPS

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

The binding activities of phage clones to the VHH were measured by phage ELISA. The OD values at 490 nm were measured and data shown were reproduced from the Fig. 2A in the reference.

Brief Description:

Bound phages were eluted with 100μl elution buffer. In order to compete with the immobilized VHH on the plate, the elution buffer was added VHH in a concentration of 100μg/ml.

BiopanningDataSet Comments:

Curator:

Curation Date:

2017-01-14

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