BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3093

Peptides:

Sequences	Affinity Values
HAIYPRH(11) NLMSRPI(10) AHQQHLR(3) ATPIHWR(2) STAPRPY(1) QFPSFTQ(1)	[0.51] [1.47] [NT] [NT] [NT] [NT]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 27720899

Target Name:

Tumor necrosis factor-α(TNF-α)

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

The absorbance was measured at 450 nm using ELISA reader. In order to calculate the binding constant (Kd), the data were analyzed using Prism program (version 6.01, Graphpad Software Inc.)

Brief Description:

BiopanningDataSet Comments:

The specific phage with the sequences of NLMSRPI (P51) and HAIYPRH (P52) have ability to specifically bind to TNF-α with Kd of 1.47 and 0.51 nM, respectively. The irrelevant phage has no significant binding affinity towards TNF-α. In addition, the MTT assay was carried out to assess the ability of the selected P51 and P52 peptides to inhibit TNF-αcytotoxicity on L929 cells either as the peptides displayed by the phage particles or the synthesized heptamer peptides. The results of MTT assay showed that P51 and P52 peptides inhibit TNF-α cytotoxicity with IC50 values of 25.15 ± 2.18 and 7.08 ± 2.24μM, respectively.

Curator:

Curation Date:

2017-01-14

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