BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3003

Peptides:

Sequences
STYTSVS(2) SYYTSVS(1) SHYTSVS(1) SYYTSVS(1) VTDTSVS(1) ATYTSVS(1) TYTSVS(1) VTYTSVS(1) SHLYYVS(1) NSPWLMT(1) PNMQSQS(1) KLRWNQT(1) YRNTPLS(1) APPPSPT(1) PPARHRC(1) APAQAKS(1) YLCGSR(1) LMIRQPD(1)

Sequences Features:

Motif:

TSVS

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 23375098

Target Name:

Helix 69 of Escherichia coli 23S rRNA

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Before the positive selection, the phage solution (10 μL of the original library in 100 μL buffer A, 2.0e11 pfu, in which pfu is plaque forming unit) was pipetted into a well having no immobilized target RNA and incubated for 1 h at RT with gentle shaking for prescreening of the library.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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