BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2961

Peptides:

Sequences

Affinity Values

NTWHNSR(12/34)
WHKEQFW(9/34)
QTKMTNP(2/34)
TVKTRPA(2/34)
AGPTRIS(2/34)
THSPGLL(1/34)
SINPDNR(1/34)
ALLADSR(1/34)
EIALGAR(1/34)
ATSAIHG(1/34)
HVQLLIF(1/34)
SITTVAA(1/34)

[0.33 ± 0.03]
[0.12 ± 0.02]
[0.20 ± 0.02]
[0.29 ± 0.02]
[0.11 ± 0.05]
[0.23 ± 0.02]
[0.20 ± 0.02]
[0.22 ± 0.04]
[0.22 ± 0.03]
[0.14 ± 0.02]
[1.89 ± 0.03]
[0.13 ± 0.02]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (competitive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 26577672

Target Name:

Immunoglobulin G (IgG) of Tuberculosis (TB) patients

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

The binding activities of the positive phage clones to IgG from TB patients were measured by phage ELISA. The A450 values were measured and data shown were reproduced from the Fig. 1a in the reference. Data are presented as the mean ± SD. The first eluted phage library was used as negative control and had the affinity value 0.39 ± 0.03 with this target.

Brief Description:

Bound phage was eluted with free purified IgG from TB patients to compete the bound phage away from the immobilized antibodies on the plate.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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