BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2921

Peptides:

Sequences	Affinity Values
PHLATLF(8/10)	[2.86 ± 0.46]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (in vivo)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 26081347

Target Name:

Ovarian cancer A2780 cell

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

FliTrx M13 phage display library (X7)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Affinities of the selected phage clones to ovarian cancer A2780 cell were measured by cell-based ELISA. The binding of each phage to the A2780 cells was compared with the original library locus coeruleus. The phage optical density ratio of >2.1 indicated that specific binding to the A2780 cells.

Brief Description:

In the initial step, a 7-mer library was injected into nude mice through a vein. After three rounds of screening, phages were enriched.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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