BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2916

Peptides:

Sequences

Affinity Values

SKVGYPT
YKASLIT
ALGGVAM
QSPRSIS
ANLWPDG
GPLSHKG
NNNWPWT
IQMTDIA
SRHLHEW
APVTSMK
ATNKITK

[0.36 ± 0.16]
[1.00 ± 0.16]
[0.66 ± 0.35]
[0.77 ± 0.06]
[0.80 ± 0.05]
[0.19 ± 0.18]
[0.70 ± 0.09]
[0.44 ± 0.30]
[0.36 ± 0.02]
[0.34 ± 0.07]
[NT]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 26346061

Target Name:

Chromium(III)

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Affinities of the selected phage clones to Cr(III) were measured by phage ELISA. The A405 values were measured and data shown were reproduced from the Fig. 2 in the reference. The library was used as controls, which had the value of 0.30 ± 0.03 when the phage library binded to the target.

Brief Description:

A complete biopanning procedure consists of prenegative screening against blank NTA resin (1 round), positive screening against Cr(III) (3 rounds), and postnegative screening against the other metal species (Iron ion : 1 round, Cadmium ion : 2 rounds, Copper ion : 2 rounds, Zinc ion : 1 round, Mercury ion: 1 round).

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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