BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2908

Peptides:

Sequences
IDNSHTH(5) SVTIHHL(1) ITLREHH(1) KMISATE(1) VKLNLSG(1) GAMSHHK(1) ETSNTRL(1) HAGFVPS(1) YHAEHFN(1) LDHKWHK(1) GLSHEIH(1) HGGVRLY(1) HAGFVPS(1) SQLPFHH(1) HLNQQNH(1) KVLHGPH(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 26177065

Target Name:

Agrobacterium vitis polygalacturonase

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Affinities of the positive phage clones to agrobacterium vitis polygalacturonase (PG) were measured by phage ELISA. The A490 values were measured and data shown were reproduced from the Fig. 2 in the reference. Data are represented as the mean of three replicates, and error bars represent the 95% confidence interval.

Brief Description:

In the first round of panning, phages were incubated with agarose beads bound with A. vitis polygalacturonase. Beads were harvested by low-speed centrifugation for 30 s, and the supernatant containing unbound phage was removed. The agarose beads were then washed 10 times with PBST and phages were eluted. In the second round, phages were incubated with blocked agarose beads containing no bound polygalacturonase to eliminate target-unspecific phage from the phage pool. The supernatant from this step was used in a second round of panning with polygalacturonase-bound beads.

BiopanningDataSet Comments:

The peptide SVTIHHLGGGS was able to reduce A. vitis polygalacturonase activity by 35% in vitro. Truncation studies showed that the IHHL motif alone is sufficient to inhibit A. vitis polygalacturonase activity.

Curator:

Curation Date:

0000-00-00

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