BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2877

Peptides:

Sequences	Affinity Values
NKELQTV(7) VKELDTR(3) AKELSTW(2)	[1.56 ± 0.2, 0.68 ± 0.18] [1.78 ± 0.32, 1.22 ± 0.16] [2.25 ± 0.23, 0.79 ± 0.32]

Sequences Features:

Motif:

K-E-L-x-T

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 26048649

Target Name:

Imidacloprid hapten IMI

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

The binding activities of positive phage clones to IMI-BSA were measured by competitive phage ELISA respectively using 0 and 1000 ng/mL of imidacloprid. The absorbance at 450 nm was measured and data shown were reproduced from the Figure 2 in the reference.

Brief Description:

The conjugate IMI-BSA was used as binding-templates to select peptides specific for imidacloprid. Bound phages in the wells were eluted. The eluted phage solutions were transferred onto wells coated with 3% BSA alone to eliminate the non-specific binding phages.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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