BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2874

Peptides:

Sequences

Affinity Values

SGVYKVAYDWQH(16)
GFPTRFEALSSN(2)
VHWDFRQWWQPS(2)
HTSSLWHLFRST(2)
GLHTSATNLYLH(1)
HSFKWLDSPRLR(1)
SGVYKVAYDGQH(1)
KASGSPSGFWPS(1)
RRVDKVQYDRQH(1)
GLHTSALSDLH(1)

[2.77 ± 0.29]
[1.57 ± 0.25]
[1.52 ± 0.1]
[2.25 ± 0.2]
[2.23 ± 0.41]
[2.57 ± 0.12]
[2.3 ± 0.18]
[3.1 ± 0.59]
[1.51 ± 0.12]
[NT]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

4, 5

Reference:

PMID: 25955351

Target Name:

Cation-independent mannose-6-phosphate receptor

Template Name:

Insulin-like growth factor II

Structure of Target-Template Complex:

2L29

2V5P

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Binding of the phages to IGF2R and BSA was examined with phage ELISA. The binding ratio OD450(IGF2R)/OD450(BSA) was calculated and data shown were reproduced from the Figure 3B in the reference. The phage with no insert peptide was served as the control, which had the value of 0.89 ± 0.11. The experiments were performed in triplicate and presented as mean ± standard deviation. NT denotes not tested.

Brief Description:

The first, third, and fifth rounds of biopanning were conducted on recombinant human IGF2R protein, while the second and fourth rounds of biopanning were conducted against rat hepatic stellate cells HSC-T6.

BiopanningDataSet Comments:

Among the identified peptide candidates, peptide VHWDFRQWWQPS shows the highest binding affinity and specificity to recombinant human IGF2R protein and HSCs. The equilibrium dissociation constant (Kd) of peptide-431 is 6.19 μM for LX-2 cells and 12.35 μM for rat hepatic stellate cells HSC-T6. Cellular uptake of peptide-431 in LX-2 cells is significantly reduced after silencing IGF2R with siRNA. Peptide VHWDFRQWWQPS also enhances the uptake of a proapoptotic peptide (KLA peptide) in LX-2 and HSC-T6 cells, indicating that peptide VHWDFRQWWQPS can be used as a targeting ligand to deliver antifibrotic agents into not only rat but also human HSCs. Dimerization of peptide VHWDFRQWWQPS further increase its binding affinity to LX-2 cells by approximately 9-fold.

Curator:

Curation Date:

0000-00-00

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