BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2871

Peptides:

Sequences

Affinity Values

AWTCSSRLCELG
AQRYCTQSVCVW
VCNEAVCYIESW
AVCDALWCAFTA
VGTCDDRWCVIR
AYNCLHPTLCLI
VCNSLWCTLPVG
QNMFCSIATKSC

[0.2 ± 0.02]
[0.56 ± 0.01]
[0.51 ± 0.02]
[0.88 ± 0.02]
[1.28 ± 0.01]
[1.22 ± 0.02]
[0.86 ± 0.02]
[0.77 ± 0.02]

Sequences Features:

Motif:

C-D-x(2)-W-C

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 25944706

Target Name:

His-tagged porcine reproductive and respiratory syndrome virus (PRRSV) nonstructuralprotein (nsp) 7

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

The binding activities of phage clones encoding peptides with high binding affinities to His-tagged nsp7 proteins were identified by ELISA. The OD values at 450 nm were measured and data shown were reproduced from the Fig. 2 in the reference. The experiments were performed in triplicate and presented as mean ± standard deviation. Uncorrelated phage was served as the control, which had the value of 0.06 ± 0.02 when binding to the hHMGR protein.

Brief Description:

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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