BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2850

Peptides:

Sequences
WHTGTPH(4) VHPHWRH(4) LHKHPTP(3) CHPHTPH(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning, competitive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 25565805

Target Name:

Mycobacterium tuberculosis (H37Rv)

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

In the first round of biopanning, inactive MTB reference strain H37Rv was used as the target. For three further rounds of biopanning, Mycobacterium smegmatis was used as the reverse screening molecule with increased stringency of the washing steps. After four rounds of screening, Clones were eluted by Mycobacterium smegmatis.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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