BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2840

Peptides:

Sequences	Affinity Values
ICARQDPAGNCS KCPSIPGAVLCV	[1.54] [1.57]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 25710003

Target Name:

Polyclonal anti-LiPA IgG

Template Name:

L. infantum chagasi Protein Antigen (LiPA)

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

LX-8 phage display library (XCX8CX)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Individual clones isolated was added to each well of microtiter plates coated with 1 μg/well of anti-LiPA IgG and anti- T. cruzi IgGs. Phages were detected using a peroxidase conjugated anti-M13 antibody (1 : 3000). Values of absorbance at 492 nm are measured. The A492 values of phages with ICARQDPAGNCS and KCPSIPGAVLCV insert for anti- T. cruzi IgGs were 0.19 and 0.19, respectively. Data shown were reproduced from graph.

Brief Description:

Anti-LiPA IgGs were coated onto a polystyrene Petri dish overnight at a concentration of 5 μg/mL for the first two rounds of panning and a concentration of 0.5 μg/mL for the last panning.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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